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All Forums > The Porn Pool > The Porn Pool Forum Page 706 > ELIZA vs PCR DNA?
AuthorPost
KatjaKassin
Senior Member


1257 Posts
5/03
Posted - Apr 15 2004 : 10:27AM
I would like to know what is the difference between an Eliza Test and the PCR test. Do both tests measure HIV antibodies in the blood?

Katja

KatjaKassin
Senior Member

1257 Posts
5/03
Posted - Apr 15 2004 : 10:35AM
I just found this on hardcoregossip:

"The HIV DNA PCR test measures something different than the HIV antibody Elisa and HIV antibody Western blot assays. The HIV DNA PCR test looks for HIV-1 DNA in the white blood cells of a person, whereas the HIV antibody Elisa and HIV antibody Western blot assays measure the immune response to the virus. If a person has a HIV DNA PCR test, the result may be positive even if insufficient antibodies are present for detection by the HIV antibody EIA"

Katja

jrv
Senior Member

1266 Posts
2/03
Posted - Apr 15 2004 : 10:35AM
ELISA tests for antibodies. The "PCR" test is tests for the virus. Both are used because no one test does everything, and no one test is best for all times in an infected person.

There is a "Western Blot" test too.

The US Center for Disease Control has good information.

barrybrn
Senior Member

1568 Posts
9/03
Posted - Apr 15 2004 : 10:40AM
Elisa Test : Looks for T-cells/Anti-bodies. Of course there is a small window where a person is infected and hasn't yet made the HIV anit-bodies to show up on a test. The standard AIDS test is an Elisa test, which tests for the antibodies your body makes for the AIDS virus. This test is the standard test used by hospitals and blood banks in America and the world. Positive tests are confirmed with a second test called the Western blot, which also analyzes the body's antibody response. The Elisa test is usually positive 6 weeks to 6 months after infection with the HIV virus.


The PCR detects very small amounts of a particular sequence of DNA, which contains the hereditary information of cells. For HIV diagnosis, the PCR test is used to detect genetic information inserted into the DNA of human cells by HIV. The test can measure levels previously considered "undetectable". The advantage of the PCR test is that it is a direct test for the presence of the AIDS virus. If you are infected, the DNA of the AIDS virus is present in your blood. Just what is PCR? PCR stands for polymerase chain reaction. PCR is a technique to look for unique DNA. PCR for the AIDS virus looks for DNA of the AIDS virus

jrv
Senior Member

1266 Posts
2/03
Posted - Apr 15 2004 : 10:42AM
Yes. And, if someone is taking the drugs then the "PCR" test may not find any virus, even though there are antibodies.

Please be careful to check everything you see on the web with the CDC site. There is a lot of bad information out there.

 
KatjaKassin
Senior Member

1257 Posts
5/03
Posted - Apr 15 2004 : 10:49AM
I was more interested in the medical method both tests refer to and your comments helped me, thanks!

Anyway, I guess there is always a certain time window until the virus/ the antibodies (may it be 30 days or 60) can be found in the blood - no matter what test you pick.

Katja




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